Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Keating
Date created: 2015-07-28 11:00:00
Date modified: 2015-08-26 08:34:45
CUP1 copper resistance gene with RBS and promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1811777_sequence (Version 1) |
Description
Metallothionein is a copper binding protein native to S. cerevisiae that gives cells resistance to toxicity by binding 11 copper atoms. It prevents copper from reacting to form toxic molecules when bound and detoxifies free radicals. The part contains the metallothionein gene CUP1, a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034).Notes
Sequence had to be prepared to be compatible with Gibson assembly after being cut from a G block.Source
S. Cerevisiae gene CUP1 - NCBI ID 856450| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_B0034 BBa_M45090 | BBa_J23100 BBa_B0034 BBa_M45090 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_M45090 | 1,35 44,55 62,247 | BBa_J23100 BBa_B0034 BBa_M45090 |