Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Keating
Date created: 2015-08-25 11:00:00
Date modified: 2015-08-26 08:41:22
codon optimized CUP1 copper resistance gene with RBS and promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1811888_sequence (Version 1) |
Description
Metallothionein is a copper binding protein native to S. cerevisiae that gives cells resistance to copper toxicity by binding 11 copper atoms. It prevents copper from reacting to form toxic molecules when bound and detoxifies free radicals. The part contains the metallothionein gene CUP1 (BBa_K1811222), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034). The CUP1 gene used in this part was codon optimized for E. coli tRNA abundance.Notes
In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3.Source
The CUP1 gene comes from NCBI ID 856450 and is native to S. cerevisiae. The gene sequence was then codon optimized for E. coli tRNA abundance.| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_B0034 BBa_K1811222 | BBa_J23100 BBa_B0034 BBa_K1811222 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1811222 | 1,35 44,55 62,247 | BBa_J23100 BBa_B0034 BBa_K1811222 |