NdeI+E0040

BBa_K1819000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1819000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lais Ribovski

Date created: 2015-09-12 11:00:00

Date modified: 2015-09-16 07:11:11

GFP coding sequence with an N-terminal linker

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1819000_sequence (Version 1)

Description

Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5???-flanked by NdeI restriction site. It allows to characterize protein expression and secretion, and may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
Such non-usual restriction site, NdeI restriction site, was placed for cloning purposes that lead to expression of recombinant protein with no additional amino acid residue(s) between its coding sequence and the fused N-terminal tag.

Notes

As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag.

Source

GFP coding sequence source is BBa_E0040 (http://parts.igem.org/Part:BBa_E0040)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GFP E0040	31,750	feature/protein, CDS
NdeI site	1,6	feature/misc, sequence_feature
binding	7,30	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1819000/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //function/reporter
• //function/reporter/fluorescence
• iGEM Parts Registry