BBa_K1819003

BBa_K1819003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1819003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lais Ribovski

Date created: 2015-09-15 11:00:00

Date modified: 2015-09-16 07:53:47

Rubber oxygenase A - rubber degrading enzyme

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1819003_sequence (Version 1)

Description

Part BBa_K1819003 corresponds to the coding sequence of rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y (Gene bank accession code: AGT20506.1 - colocar link: http://www.ncbi.nlm.nih.gov/protein/AGT20506.1).

Notes

Because Xanthomonas sp. RoxA is exported to the extracellular environment, its open reading frame contains a sequence that codes to a signal peptide flanking its 5??? terminus. We detected this region (amino acid residues from 1 to 20) using signal peptide predictor server (4) (colocar link - http://www.compgen.org/tools/PRED-TAT) and eliminated it to generate this part with the coding sequence of RoxA flanked by SacI and NdeI restriction sites.

Also, roxA contained a EcoRI restriction site. We perform directed mutagenesis PCR (for restriction site elimination) using a plasmid template for restriction site elimination

Source

Xanthomonas sp. strain 35Y

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RoxA	7,1948	feature/protein, CDS
t-->c	124,124	sequence_alteration, feature/mutation
ScaI site	1,6	feature/misc, sequence_feature
NdeI site	1948,1954	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1819003/1

Member of these Collections

• iGEM Parts Registry