BBa_K182001

BBa_K182001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K182001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel J. Leirer, Md. Risat ul Haque

Date created: 2009-07-05 11:00:00

Date modified: 2015-05-08 01:11:06

Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K182001_sequence (Version 1)

Description

This is an intermediate part using the biobrick BBa_I732820 (RBS, LacI, Term) upstream and the Biobrick BBa_R0040 (TetR repressible promoter) downstream.

It comes with a ribosome binding site upstream of LacI and a Terminator downstream (BBa_B0015) There is no Promoter upstream of LacI, meaning that LacI will not be expressed in this piece.

The TetR repressible promoter BBa_R0040 is downstream of the Terminator.

Notes

We used two parts from the registry of standard parts.
BBa_I732820 is the upstream part and BBa_R0040 is the downstream part.

Source

BBa_I732820, BBa_R0040

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_I732100	LacI
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0040	p(tetR)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_I732100	19,1104	LacI
BBa_B0010	1113,1192	BBa_B0010
BBa_B0012	1201,1241	BBa_B0012
BBa_R0040	1250,1303	p(tetR)

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K182001/1

Member of these Collections

• iGEM Parts Registry