Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel J. Leirer, Md. Risat ul Haque
Date created: 2009-07-05 11:00:00
Date modified: 2015-05-08 01:11:06
Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K182002_sequence (Version 1) |
Description
This is an intermediate part using the biobrick BBa_R0051 as the upstream part and BBa_S03518 as the downstream part.BBa_R0051 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.
BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040.
aTc (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription.
This part comes without a terminator.
Notes
We used two parts from the registry of standard parts. BBa_R0051 is the upstream part and BBa_S03518 is the downstream part.Source
BBa_R0051, BBa_C0040| Access | Instance | Definition |
| public public | BBa_R0051 BBa_S03518 | cI lam BBa_S03518 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0051 BBa_S03518 | 1,49 58,760 | cI lam BBa_S03518 |