BBa_K182002

BBa_K182002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K182002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel J. Leirer, Md. Risat ul Haque

Date created: 2009-07-05 11:00:00

Date modified: 2015-05-08 01:11:06

Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K182002_sequence (Version 1)

Description

This is an intermediate part using the biobrick BBa_R0051 as the upstream part and BBa_S03518 as the downstream part.

BBa_R0051 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.

BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040.
aTc (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription.

This part comes without a terminator.

Notes

We used two parts from the registry of standard parts. BBa_R0051 is the upstream part and BBa_S03518 is the downstream part.

Source

BBa_R0051, BBa_C0040

Components

Access	Instance	Definition
public	BBa_R0051	cI lam
public	BBa_S03518	BBa_S03518

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051	1,49	cI lam
BBa_S03518	58,760	BBa_S03518

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K182002/1

Member of these Collections

• iGEM Parts Registry