Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MD. RISAT UL HAQUE, Daniel J. Leirer
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:06
Lambda CI promoter (BBa_R0051) with a point mutation in -35 region.
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K182004_sequence (Version 1) |
Description
The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. This particular promoter has got a point mutation ( C instead of T) in the -35 region of the promoter binding site.Notes
This part was used as an upstream part to construct the Biobruck BBa_K182005Source
BBa_R0051| Sequence Annotation | Location | Component / Role(s) |
| BBa_K182004 -35 -10 OR2 OR1 Point Mutation | 1,49 15,20 37,43 1,19 25,42 15,15 | feature/BioBrick engineered_region feature/promoter promoter feature/promoter promoter feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding point_mutation feature/s_mutation |