BBa_K1823001

BBa_K1823001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1823001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Isyatul Azizah

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 01:11:54

EDE1C8 TEV scFv

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1823001_sequence (Version 1)

Description

EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.

To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock.
You can collect the antibody by purification using Nickel column, it has Histidine (6X), and cut the target protein from another peptide using TEV protease.

This scFv antibody has Myc as the linker between the light chain and heavy chain.

Notes

OsmY, GSGG, Histidine (6X), TEV, vL, Myc, vH, terminator

Source

gene fragment synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TEV	316,334	sequence_feature, feature/misc
osmY	82,283	feature/cds, CDS
GSGG	284,296	feature/misc, sequence_feature
Histidine	297,315	tag, feature/tag
vH	335,706	CDS, feature/cds
Myc	707,737	tag, feature/tag
vL	738,1444	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1823001/1

Member of these Collections

• iGEM Parts Registry