BBa_K1823002

BBa_K1823002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1823002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Isyatul Azizah

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 01:56:04

EDE1C8 scFv

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1823002_sequence (Version 1)

Description

EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.

To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock.


This scFv antibody has Myc as the linker between the light chain and heavy chain.

Notes

osmY, GSGG, Histidine (6X), vH, Myc,vL, terminator

Source

gene fragment synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
osmY	7,610	feature/cds, CDS
GSGG	611,623	feature/misc, sequence_feature
Histidine	624,642	tag, feature/tag
vH	642,1005	CDS, feature/cds
Myc	1006,1036	sequence_feature, feature/misc
vL	1037,1361	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1823002/1

Member of these Collections

• iGEM Parts Registry