BBa_K1823003

BBa_K1823003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1823003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Isyatul Azizah

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 02:25:37

EDE1C8 + promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1823003_sequence (Version 1)

Description

EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.
It has promoter and RBS on the upstream site, also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock. You can collect the antibody by purification using Nickel column, it has Histidine (6X).

Note:
This part does not have TEV sequence to cleave the targeted antibody and separate the antibody from another prtoein.
This scFv antibody has Myc as the linker between the light chain and heavy chain.

Notes

osmY, GSGG, Histidine (6X), vH, Myc, vL

Source

gene fragment synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
osmY	24,627	feature/cds, CDS
GSGG	628,630	feature/misc, sequence_feature
Histidine	631,649	tag, feature/tag
vH	650,1013	feature/cds, CDS
Myc	1014,1044	feature/tag, tag
vL	1045,1369	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1823003/1

Member of these Collections

• iGEM Parts Registry