Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-06 05:22:36
pBAD + RNA Thermometer U6
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1824005_sequence (Version 1) |
Description
This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer U6 BBa_K1824563 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequenceSource
U6 was from registry.| Access | Instance | Definition |
| public public | BBa_I13453 BBa_K1824563 | BBa_I13453 BBa_K1824563 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K1824563 | 1,130 131,197 | BBa_I13453 BBa_K1824563 |