| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K1824555_sequence (Version 1)
|
Description
This was a mutated pLux that permitted ideal ON/OFF ratio based on the original promoter Lux pR (BBa_R0062). BBa_R0062 was believed to have high constitutative expression in the absent of 3-oxo-hexanoyl-HSL and this problem went serious when regulatory promoter was coupled with toxins or other repression/activation parts. By using this mutated pLux, genetic circuits would be more easily to control and more sensitive to external signal change.
Notes
Due to the consideration of binding efficency of LuxR-AHL complex to the Lux box that embedded in pLux. Zhang only mutated -10 region of the original pLux.
Source
The DNA sequences of this part was provided by Haoqian Zhang, Peking University.
