BBa_K1824561

BBa_K1824561 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1824561

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wenbo Xu

Date created: 2015-09-05 11:00:00

Date modified: 2015-09-06 05:30:05

RNA Thermometer FourU (Specially designed for T7)

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K1824561_sequence (Version 1)

Description

This is a specially designed FourU RNA thermometer that with a unique spacer at the front, which would make it compatible with T7 BBa_I13453.

FourU RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation (Figure 2). The melting temperature of this RNA thermometer is 37 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process.

Notes

To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.

Source

FourU was previously submitted by TuDelft 2008.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1824561/1

Member of these Collections

• iGEM Parts Registry