Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Altynay Abdirakhmanova
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-09 12:50:36
PnlmAB+sgRNA for VicK
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1828901_sequence (Version 1) |
Description
This composite part consists form nlmAB promoter and sequence for sgRNA for VicK. nlmAB promoter is a natural promoter found in Streptococcus mutans. This promoter is upregulated by addition of Competence Stimulating Peptide (CSP). VicK was found to be responsible for lactic acid production and acid tolerance of Streptoccoccus mutans. sgRNA for VicK is designed to specifically target VicK in the genome of S.mutans with deadCas9 that lack endonuclease activity.Notes
Design of sgRNA for VicK was done according to the Nature protocolSource
nlmAB promoter sequence is a genomic sequence of S.mutans.| Access | Instance | Definition |
| public public | BBa_K1828999 BBa_K1828990 | BBa_K1828999 BBa_K1828990 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1828999 BBa_K1828990 | 1,121 130,232 | BBa_K1828999 BBa_K1828990 |