Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Konstantin Borisov
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 08:02:07
pProt-GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1833001_sequence (Version 1) |
Description
This part produces GFP in the presence of sigma-70 E. coli RNA polymerase; however, the promoter contains two sites for a synthetic repressor to bind. Without the presence of this repressor, the part can serve as a strong constitutive promoter, as shown in the pictures below. For more information on the repressor, visit http://2015.igem.org/Team:Pitt/Protease/Project.The GFP produced is a mutant known as GFPmut3b, and the original citation is as follows:
Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996).
(http://www.sciencedirect.com/science/article/pii/0378111995006850)
It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040.
Notes
The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.Source
The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The pProt promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part.| Access | Instance | Definition |
| public public | BBa_K1833998 BBa_E0840 | BBa_K1833998 GFP genera |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1833998 BBa_E0840 | 1,53 54,931 | BBa_K1833998 GFP genera |