| Types | DnaRegion
|
| Roles | engineered_region
Generator
|
| Sequences | BBa_K1833006_sequence (Version 1)
|
Description
This part generates a fusion protein containing the Zif268 (Zinc finger 225 gene) DNA binding domain fused to Gal11P, as used in the yeast 2-hybrid system. This part can be used in combination with BBa_K1833004 to create an E. coli 2-hybrid system. With a promoter containing the Zif268 binding sequence, these two parts can combine to recruit RNA polymerase to a weak promoter, such as the one in BBa_K1833007. Such a system was used in Joung, J. K. et. al. "A bacterial two-hybrid selection system for studying protein???DNA and protein???protein interactions," PNAS, 2000, vol. 97, no. 13.
Notes
A nucleotide between the promoter and RBS had to be mutated to remove an illegal restriction site.
Source
Joung, J. K. et. al. "A bacterial two-hybrid selection system for studying protein???DNA and protein???protein interactions," PNAS, 2000, vol. 97, no. 13.
