Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Connor McFadden
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-25 09:47:59
Glucose Sensitive Promoter via MLC E. coli interactions
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1838000_sequence (Version 1) |
Description
This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found upstream of the base promoter used for construction, BBa_J23119.Notes
One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4).Source
1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print.2. http://parts.igem.org/Part:BBa_J23119
| Sequence Annotation | Location | Component / Role(s) |
| MLC Binding Site BBa_J23119 | 1,23 24,58 | operator feature/operator feature/promoter promoter |