BBa_K1840002

BBa_K1840002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1840002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Anna Adrian

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 01:47:02

Zwf promotor from Pseudomonas putida

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1840002_sequence (Version 1)

Description

This basic part is the promotor in front of the gene for zwf (glucose 6-phosphate dehydrogenase) in Pseudomonas putida. Zwf is concerned with the glucose metabolism in P. putida. The zwf promotor is negatively regulated by the repressor called HexR. It has a binding site for glucose derivative 2-keto-3-deoxy-6-phosphogluconate (KDPG) and binding leads to the release from the promotor.
For our project (NTNU Trondheim 2015) we integrated the promotor in front of the mCherry gene. Hence we built a glucose detection device, that expresses mCherry according to the glucose (and therefore KDPG) level in the environment.
Note: The promotor edd (BBa_K1840000) is repressed by the same repressor HexR. See biobrick parts BBa_K1840001 and BBa_K1840003 for other promotors on operons regulated by glucose derivatives.

Notes

The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the zwf gene. We assumed it starts directly after the previous gene. This gene codes for the repressor, HexR, and is situated on the opposite strand.

Source

The part was designed after information from the paper by DADDAOUA, A., KRELL, T. & RAMOS, J.-L. 2009. Regulation of Glucose Metabolism in Pseudomonas: The Phosphorylative Branch and Entner-Doudoroff Enzymes are Regulated by a Repressor Containing a Sugar Iisomerase Domain. Journal of Biological Chemistry, 284, 21360-21368.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1840002/1

Member of these Collections

• iGEM Parts Registry