Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Anna Adrian
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 02:16:57
Device: TT-zwf-mCherry
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1840007_sequence (Version 1) |
Description
This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the promotor zwf is influenced by it. The zwf promotor (BBa_K1840002) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme zwf (glucose 6-phosphate dehydrogenase). The repressor HexR can bind the zwf promotor sequence and thus prevent the transcription of the zwf gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds to the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene(s) downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.Notes
As promotor sequence, we assumed all DNA in front of the zwf gene up to the next gene, coding for the repressor HexR. Since it is encoded on the opposite strand, there is the possibility, that zwf functions as a promotor for this gene as well. Therefore the double terminator was needed.Source
The double terminator was already registered in the iGEM catalogue. The zwf promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.| Access | Instance | Definition |
| public public public | BBa_B0014 BBa_K1840002 BBa_K1840004 | BBa_B0014 BBa_K1840002 BBa_K1840004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0014 BBa_K1840002 BBa_K1840004 | 1,95 104,305 312,1025 | BBa_B0014 BBa_K1840002 BBa_K1840004 |