Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Walter Thavarajah
Date created: 2015-08-16 11:00:00
Date modified: 2015-08-17 06:19:14
LuxCDED
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1842002_sequence (Version 1) |
Description
The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. A duplicate copy of LuxD is present, resulting in its overexpression. Genes used are codon optimized for expression in yeast.Notes
The coding sequence was too large to be synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly.Source
Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum.| Sequence Annotation | Location | Component / Role(s) |
| LuxC FLAG P2A Linker LuxD V5 P2A Linker LuxE HA P2A Linker LuxD (Duplicate) V5 Stop x2 | 1,1434 1435,1458 1459,1524 1525,2442 2443,2484 2485,2550 2551,3669 3670,3702 3703,3768 3769,4686 4687,4728 4729,4734 | CDS feature/protein feature/tag tag feature/misc sequence_feature feature/protein CDS feature/tag tag sequence_feature feature/misc CDS feature/protein feature/tag tag sequence_feature feature/misc feature/protein CDS tag feature/tag stop_codon feature/stop |