BBa_K1842003

BBa_K1842003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1842003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Walter Thavarajah
Date created: 2015-08-16 11:00:00
Date modified: 2015-08-17 06:32:01

LuxCDEE



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1842003_sequence (Version 1)

Description

The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. A duplicate copy of LuxE is present, resulting in its overexpression. Genes used are codon optimized for expression in yeast.

Notes

The coding sequence was too large to be synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly.

Source

Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum.

Sequence Annotation Location Component / Role(s)
LuxC
FLAG
P2A Linker
LuxD
V5
P2A Linker
LuxE
HA
P2A Linker
LuxE (duplicate)
HA
Stop x2
1,1434
1435,1458
1459,1524
1525,2442
2443,2484
2485,2550
2551,3669
3670,3702
3703,3768
3769,4887
4888,4920
4921,4926
CDS feature/protein
feature/tag tag
sequence_feature feature/misc
feature/protein CDS
tag feature/tag
feature/misc sequence_feature
CDS feature/protein
tag feature/tag
feature/misc sequence_feature
CDS feature/protein
tag feature/tag
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1842003/1