Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Walter Thavarajah
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 08:51:03
LuxDEC
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1842004_sequence (Version 1) |
Description
The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. One copy of each gene is present. Genes used are codon optimized for expression in yeast.Notes
The part was PCR amplified from BBa_K1842001.Source
Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum.| Sequence Annotation | Location | Component / Role(s) |
| LuxD V5 Tag P2A LuxE HA Tag P2A LuxC FLAG Tag Stop x2 | 1,918 919,960 961,1026 1027,2145 2146,2178 2179,2244 2245,3678 3679,3702 3703,3708 | CDS feature/protein feature/tag tag feature/misc sequence_feature CDS feature/protein tag feature/tag sequence_feature feature/misc CDS feature/protein tag feature/tag stop_codon feature/stop |