Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luba Prout
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-19 11:59:35
stf (short tail fibre) gene of bacteriophage lambda
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1846004_sequence (Version 1) |
Description
The stf gene encodes the short tail fibres found on the wild-type strain of bacteriophage lambda. The gene has been inactivated by a single frameshift mutation in the lab strain most commonly used in research (λ-PaPa). This gene has been created using the ORF-314 and ORF-401, cloning the two open reading frames into a single in-frame coding sequence through the use of an Eco-RV restriction site.The C-terminal end of the stf protein offers host receptor recognition for OmpC on the surface of E.coli, widening the host range of λ-PaPa, which recognises only the lamB protein through its tip attachment protein. Additionally, this gene displays a high level (~40%) of homology with the gp37 of bacteriophage T4.