BBa_K1850003

BBa_K1850003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1850003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lydia Goldberg
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 07:34:47

pRha - fimH - SpyTag_225 - HisTag_258



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1850003_sequence (Version 1)

    Description

    Titratable Rhamnose promtoter with fimH gene and HisTag insertion at position 258 for nickel binding. For production of modified type 1 pili.

    Notes

    edited out illegal cut sites

    Source

    fish comes from E. coli K-12 genome

    Sequence Annotation Location Component / Role(s)
    pRha
    fimH
    HisTag
    SpyTag
    B0034
    		4,243
    270,1229
    1143,1160
    1005,1043
    252,263
    		promoter feature/promoter
    CDS feature/protein
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    ribosome_entry_site feature/rbs

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1850003/1
     

    Attachments

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