BBa_K1850003

BBa_K1850003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1850003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lydia Goldberg
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 07:34:47

pRha - fimH - SpyTag_225 - HisTag_258



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1850003_sequence (Version 1)

Description

Titratable Rhamnose promtoter with fimH gene and HisTag insertion at position 258 for nickel binding. For production of modified type 1 pili.

Notes

edited out illegal cut sites

Source

fish comes from E. coli K-12 genome

Sequence Annotation Location Component / Role(s)
pRha
fimH
HisTag
SpyTag
B0034
4,243
270,1229
1143,1160
1005,1043
252,263
promoter feature/promoter
CDS feature/protein
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
ribosome_entry_site feature/rbs
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1850003/1