Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iain Galpin
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:11:52
Lpp-OmpA sZF10
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1855002_sequence (Version 1) |
Description
A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF10),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli.Notes
Lpp-OmpA is originally from E.coli; sZF10 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.htmlSource
We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF10 domain is preceeded by an NheI cut site and followed by a ClaI cut site.| Sequence Annotation | Location | Component / Role(s) |
| pL_lac B0034 Strong RBS Lpp-OmpA Linker sZF10 Linker 3x FLAG BBa_K864600 T0 Terminator | 1,56 60,80 84,518 522,557 564,833 840,863 867,938 945,996 | feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc CDS feature/protein feature/misc sequence_feature feature/tag tag feature/stop stop_codon |