Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iain Galpin
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:13:15
BclA-Zif268
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1855005_sequence (Version 1) |
Description
A fusion protein of a transmembrane domain (BclA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.Notes
We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The BclA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.Source
BclA is originally from Bacillus Anthracis; Zif268 is from mice.| Sequence Annotation | Location | Component / Role(s) |
| pL_lac B0034 Strong RBS BclA Linker Zif268 Linker 3x FLAG BBa_K864600 T0 Terminator | 1,56 60,80 84,146 150,185 192,458 465,488 492,563 570,621 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/protein CDS feature/misc sequence_feature tag feature/tag stop_codon feature/stop |