BBa_K1859001

BBa_K1859001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1859001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wataru Fukuda
Date created: 2015-09-06 11:00:00
Date modified: 2015-09-18 01:55:27

the 3??side of the intron[BBa_K1332005] +GGSGGS linker



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1859001_sequence (Version 1)

Description

This part consists of mRNA circularization device (5?? side) and linker (amino acid sequence: GGSGGS). It???s the improved mRNA circularization parts (5?? side). The linker keeps a function of coded protein that is translated semi-permanently. The amino acids that consist of the linker is glycine and serine. Because their steric hindrance are small and their hydrophilicity are strong. The protein coding sequence that is inserted between this device and mRNA circularization device (3??? side) can be circularized. If you circularized the protein coding sequence (Its a stop codon have been removed.) with mRNA circularization device (3?? side) (endless translation)(BBa_K1332009), you can get a circular mRNA that is translated semi-permanently.

Notes

We built a sequence of linker(GGSGGS) into the circuler mRNA. The linker keeps a function of coded protein that is translated semi-permanently.

Source

T4 phage

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BBa_K1859001/1