| Types | DnaRegion
|
| Roles | mature_transcript_region
RNA
|
| Sequences | BBa_K1859010_sequence (Version 1)
|
Description
This part consists of mRNA circularization device (5?? side) and linker (amino acid sequence: GGSGGSGGSGGSGGSGGS). It???s the improved mRNA circularization parts (5?? side). The linker may keep a function of coded protein that is translated semi-permanently. The amino acids that consist of the linker are glycine and serine. Because their steric hindrance is small and their hydrophilicity are strong. The protein coding sequence that is inserted between this device and mRNA circularization device (3??? side) can be circularized. If you circularized the protein coding sequence (Its a stop codon have been removed.) with mRNA circularization device (3?? side) (endless translation)(BBa_K1332009), you can get a circular mRNA that is translated semi-permanently.
Notes
We built a sequence of linker (GSGSGSGSGSGSGSGSGS) into the circuler mRNA. The linker may keep a function of coded protein that is translated semi-permanently.
Source
T4 phage
