Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yusuke Banno
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-17 08:34:00
Histidine tag and RFP linker [GGSGGS*3] semi-permanent generator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1859022_sequence (Version 1) |
Description
This device is all-in-one mRNA circularization device. If you transfer this device into E. coli, you can get a protein (its sequence is ???GGSGGSGGSGGSGGSGGS - amino acid derived from RBS sequence - HisRFP ??? GGSGGSGGSGGSGGSGGS - amino acid derived from ribozyme sequence ??? GGSGGSGGSGGSGGSGGS - amino acid derived from RBS sequence -???)Notes
This part includes sequences which one of the linkers we designed(GGSGGSGGSGGSGGSGGS).Source
T4 phage| Access | Instance | Definition |
| public public public public public public | BBa_R0010 BBa_K1859009 BBa_B0034 BBa_K1332002 BBa_K1859013 BBa_B0015 | LacI BBa_K1859009 BBa_B0034 BBa_K1332002 BBa_K1859013 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1859009 BBa_B0034 BBa_K1332002 BBa_K1859013 BBa_B0015 | 1,200 209,456 465,476 485,1187 1196,1367 1376,1504 | LacI BBa_K1859009 BBa_B0034 BBa_K1332002 BBa_K1859013 BBa_B0015 |