Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Avril von Hoyningen-Huene, Sindhu Thiagarajan, Verena Siebert
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 01:16:10
eforRed_BCEL
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1865001_sequence (Version 1) |
Description
eforRed_BCEL consists of the dockerin BCEL (BBa_K1865000) and eforRed (BBa_K592012). By adding our dockerin to this BioBrick from the Uppsala 2011 team, we have made it compatible for use with our scaffoldin. The eforRed that will bind to the equivalent cohesin on a scaffoldin via the dockerin connected to it. The dockerin is derived from Bacteroides cellulolyticus and binds to the cohesin consensus sequence from this organism.eforRed was codon optimized for use in E.coli when used by Uppsala in 2011. It originally stems from
eforRed_BCEL can used as a reporter for the detection of the scaffoldin construct in a sample.
Notes
The Composite BioBrick was assembled with alternative restriction sites into our expression vector pBAD. In this BioBrick the two sequences can be separated into their composite parts by using KpnI to cut in between the two. The outer restriction sites are EcoRI (prefix) and PstI (Suffix).Source
BCEL was isolated from the DNA of Bacteroides cellulolyticus.eforRed was used from a gBlock with the BioBrick sequence of BBa_K592012.
| Access | Instance | Definition |
| public public | BBa_K1865000 BBa_K592012 | BBa_K1865000 eforRed |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1865000 BBa_K592012 | 1,254 261,941 | BBa_K1865000 eforRed |