Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Oehler
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 04:54:18
Promoter+RibosomeBindingSite+NosZ+SuperYellowFluorescenceProtein (in pSB1A3)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1866004_sequence (Version 1) |
Description
This part catalyses the conversion of Nitrous Oxide to Nitrogen gas.This part consists of a Super Yellow Fluorescence Protein tag to check the expression of the NosZ gene.
Notes
The NosZ gene has a PstI enzyme site within its sequence. This is why the part is not 3A assembly compatible.We used an alternate strategy to ligate the Promoter+RibosomeBindingSite construct upstream to the NosZ+SYFP construct in its native backbone.
Source
The Promoter+RibosomeBindingSite part was obtained from the biobrick BBa_K880005.The NosZ+SYFP construct was used from the biobrick BBa_K1866001.
| Access | Instance | Definition |
| public public | BBa_K880005 BBa_K1866001 | BBa_K880005 NosZ+SYFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K880005 BBa_K1866001 | 1,55 62,1972 | BBa_K880005 NosZ+SYFP |