Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Conary Meyer
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 12:49:32
SAM Synthetase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1869001_sequence (Version 1) |
Description
The S-adenosylmethionine (SAM) Synthetase is a cytosolic protein the is responsible for catalyzing the reaction between methionine and ATP to produce SAM. SAM functions as the primary methyl donor in numerous methylation reactions. It also plays a major role in in the SAM cycle and cysteine biosynthesis. This protein can be used in conjunction with other proteins to increase the concentration of methyl donors in a culture to help drive forward reactions. When used in conjunction with BBa_K1869000, the CFA synthase, SAM drives the CFA synthesis reaction forward.Notes
This sequence was extracted through PCR with over hangs designed to ligate into the pSB1C3 vector using the PIPE cloning method.Source
The SAM synthetase sequence was extracted, through PCR, from E. coli K-12 TG1. This protein is inherent in various organisms as it plays a major role in methionine and cysteine biosynthesis.| Sequence Annotation | Location | Component / Role(s) |
| SAM Synthetase | 1,1158 | feature/cds CDS |