BBa_K1871001

BBa_K1871001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1871001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Broad Run High School iGEM Team

Date created: 2015-09-12 11:00:00

Date modified: 2015-12-17 01:16:23

Alpha amylase with native secretion

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1871001_sequence (Version 1)

Description

This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence (Part BBa_K165002) the ADH1 terminator (Part BBa_K392003)
. Expression is controlled by the cyc yeast promoter (Part BBa_K105027).

Notes

TBD

Source

The amylase gene was obtained from the published sequence for Bacillus amyloliquefaciens

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Minimal cyc Promoter	1,104	feature/promoter, promoter
Kozak Sequence	105,125	ribosome_entry_site, feature/rbs
Native Alpha Amylase Secretion Sequence	126,215	feature/tag, tag
Alpha Amylase Coding Sequence	216,1667	CDS, feature/cds
ADH1 Terminator	1668,1855	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1871001/1

Member of these Collections

• //chassis/eukaryote/yeast
• iGEM Parts Registry