Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: shau-yi wu
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:13
RBS+lacI repressor from E. coli (+LVA)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K188122_sequence (Version 1) |
Description
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.