BBa_K188123

BBa_K188123 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K188123
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: shau-yi wu
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:13

LacI(LVA)+RBS



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K188123_sequence (Version 1)

Description

lacI repressor from E. coli (+LVA)
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

And a RBS

Notes

NONE

Source

NONE

Access Instance Definition
public
public
BBa_C0012
BBa_B0034
lacI
BBa_B0034
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K188123/1