BBa_K1886009

BBa_K1886009 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1886009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lejian Jiang

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-17 11:26:09

AND GATE-output-2

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1886009_sequence (Version 1)

Description

This is a report system to test the AND GATE made up of SupD tRNA coding gene and T7 polymerase with two amber mutations. T7 promoter is a very specific promoter which is transcribed only by specific T7 RNA polymerase. Following the PT7 promoter is the reporter gene RFP, Only when the two inputs are given, the RFP gene can be expressed.

Notes

PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.

Source

This part is obtained from iGEM DNA Distribution kits, and is created by iGEM PKU Team in 2009.

Components

Access	Instance	Definition
public	BBa_K1321338	BBa_K1321338
public	BBa_E1010	mRFP1
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1321338	1,43	BBa_K1321338
BBa_E1010	50,755	mRFP1
BBa_B0015	764,892	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1886009/1

Member of these Collections

• iGEM Parts Registry