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BBa_K188713

BBa_K188713 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K188713

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hsiao-Yun Ko

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:13

GFP regulated by tetracycline, weaker GFP expression.

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K188713_sequence (Version 1)

Description

This device modify from BBa_K145279,design by iGEM08_KULeuven. It consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracycline (or anhydrotetracycline ). This modified device use Degenerated PCR to make different TATA box at -10 sequences. This composite is similar to BBa_K188707, but the GFP strength is lighter than BBa_K188707.

Notes

Source

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_J23116 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_K188733 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	BBa_J23116 BBa_B0034 tetR BBa_B0010 BBa_B0012 BBa_K188733 BBa_B0032 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23116 BBa_B0034 BBa_C0040 BBa_B0010 BBa_B0012 BBa_K188733 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	1,35 44,55 62,746 755,834 843,883 892,945 954,966 973,1692 1701,1780 1789,1829	BBa_J23116 BBa_B0034 tetR BBa_B0010 BBa_B0012 BBa_K188733 BBa_B0032 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K188713/1