Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lycka Kamoen, Maria Vazquez
Date created: 2016-09-07 11:00:00
Date modified: 2016-10-16 01:23:05
Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1890002_sequence (Version 1) |
Description
Silicatein, originating from the demosponge Tethya aurantia, catalyzes the formation of polysilicate. As described by Curnow et al, the silicatein gene was fused to the transmembrane domain of outer membrane protein A (OmpA), in order to display it at the surface of the cell. The fusion of silicatein and OmpA is constructed according to Francisco et al, consisting of the transmembrane domain of OmpA together with the signaling peptide and the first nine N-terminal amino acids of lipoprotein (Lpp). The coding sequence in this BioBrick is combined with the strong RBS.Notes
In frame fusion, codon optimized for E. coli.Source
Tethya aurantia| Access | Instance | Definition |
| public public public public public | BBa_G0002 BBa_B0034 BBa_G0000 BBa_K1890052 BBa_K1890051 | SX scar BBa_B0034 scar BBa_K1890052 BBa_K1890051 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_G0002 BBa_B0034 BBa_G0000 BBa_K1890052 BBa_K1890051 | 1,8 9,20 21,26 27,488 489,1481 | SX scar BBa_B0034 scar BBa_K1890052 BBa_K1890051 |