BBa_K1891000

BBa_K1891000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1891000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhao Jingyu

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 01:36:56

alpha-tubulin

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1891000_sequence (Version 1)

Description

It encodes alpha-tubulin from human breast cancer cell line Mcf-7. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of alpha-tubulin

Notes

To get this part, we extracted human breast cancer cell line Mcf-7 cDNA. And then we designed primers to get this gene fragment with prefix and suffix. Because the sequence of alpha -tubulin have tow EcoRI restriction enzyme cutting sites. So we use point mutation to mutate these sites.

Source

We get this gene from human breast cancer cell line Mcf-7 cDNA using PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
alpha-tubulin	1,1356	feature/misc, sequence_feature

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1891000/1

Member of these Collections

• iGEM Parts Registry