Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 01:46:35
beta-tubulin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1891001_sequence (Version 1) |
Description
It encodes beta-tubulin from human breast cancer cell line Mcf-7. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of beta-tubulin.Notes
To get this part, we extracted human breast cancer cell line Mcf-7 cDNA. And then we designed primers to get this gene fragment with prefix and suffix. Because the sequence of beta -tubulin has a PstI restriction enzyme cutting site. So we use point mutation to mutate these sites.Source
We get this gene from human breast cancer cell line Mcf-7 cDNA using PCR.| Sequence Annotation | Location | Component / Role(s) |
| beta-tubulin | 1,1335 | sequence_feature feature/misc |