BBa_K1891006

BBa_K1891006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1891006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 09:27:47

C-Luciferase+alpha-tubulin



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1891006_sequence (Version 1)

Description

It expresses C-Luciferase-alpha-tubulin fusion protein. The C-terminal of C-Luciferase links to the N-terminal of alpha-tubulin. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.

Notes

We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of C-Luciferase and a sense primer of alpha-tubulin with a linker about 42bp. And then doing fusion PCR.

Source

C-Luciferase comes from pCambia1300-C-Luciferase plasmid. Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA.

Sequence Annotation Location Component / Role(s)
C-luciferase
linker
alpha-tubulin
1,459
459,501
502,1857
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1891006/1