BBa_K1891008

BBa_K1891008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1891008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 10:32:28

alpha-tubulin+YCE



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1891008_sequence (Version 1)

Description

This part expresses alpha-tubulin-YCE fusion protein. The C-terminal of alpha-tubulin links to the N-terminal of YCE. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.

Notes

We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and YCE gene fragment. And then we redesigned a antisense primer ofalpha-tubulin and a sense primer of YCE with a linker about 42bp. And then doing fusion PCR.

Source

Alpha-tubulin comes from human breast cancer cell line Mcf-7. YCE comes from pSPYCE plasmid.

Sequence Annotation Location Component / Role(s)
alpha-tubulin
YCE
linker
1,1356
1399,1650
1357,1398
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1891008/1