BBa_K1896002

BBa_K1896002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-14 05:25:33

inaZ (GG safe)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1896002_sequence (Version 1)

Description

This is an improved version of [[Part:BBa_K584021|BBa_K584021]], which encodes the ice nucleating protein gene inaZ from Pseudomonas syringae. Two BsaI sites have been removed to make the part safe for Golden Gate cloning.

Notes

The many repeats in this gene seem to make this sequence virtually impossible to synthesize, even after changing the codons used. Over-expression of this part at high levels is toxic.

Source

This part was based on [[Part:BBa_K584021|BBa_K584021]].

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
inaZ	1,3603	CDS, feature/cds
start	1,3	start_codon, feature/start
stop	3601,3603	feature/stop, stop_codon
repeat region	526,3453	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896002/1

Member of these Collections

• iGEM Parts Registry