BBa_K1896004

BBa_K1896004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 05:41:27

linker-Streptavidin-His (tetrameric)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1896004_sequence (Version 1)

Description

This is an improved version of [[Part:BBa_K283010|Part:BBa_K283010]], which encodes for the core Streptavidin protein of Streptomyces avidinii. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.

Notes

One BsaI site was removed to enable Golden Gate cloning.

Source

[[Part:BBa_K283010|Part:BBa_K283010]]

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GSTGS linker	1,15	CDS, feature/cds
BamHI site removed	60,60	feature/s_mutation, point_mutation
Streptavidin	16,396	CDS, feature/cds
His-tag	397,414	CDS, feature/cds
stop	415,420	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896004/1

Member of these Collections

• iGEM Parts Registry