Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bob Van Hove, Maarten Van Brempt
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 05:42:05
mGFPuv2 (N-part)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1896006_sequence (Version 1) |
Description
Variant of [[Part:BBa_K1896001|Part:BBa_K1896001]] that lacks stop codons so it can be used in N-terminal protein fusions.mGFPuv2 contains the following mutations:
- F99S/M153T/V163A: GFPuv or cycle 3 GFP was optimised for excitation by UV light (360-400nm). [1]
- S208L: Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2]
- A206K: Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]
Notes
The stop codon was removed to enable scarless N-terminal protein fusions.Source
We started from pBAD-GFPuv (GenBank: U62637.1, Clontech), applied the described mutations and codon optimised the sequence for E. coli.| Sequence Annotation | Location | Component / Role(s) |
| mGFPuv2 F99S M153T V163A A206K S208L start | 1,714 295,297 457,459 487,489 616,618 622,624 1,3 | CDS feature/cds sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation feature/mutation sequence_alteration start_codon feature/start |