BBa_K1896007

BBa_K1896007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1896007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bob Van Hove, Maarten Van Brempt

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 05:45:10

mGFPuv2 (C-part)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1896007_sequence (Version 1)

Description

Variant of [[Part:BBa_K1896001|Part:BBa_K1896001]] where the start codon was replaced by a GSTGS linker so it can be used in C-terminal protein fusions.

mGFPuv2 contains the following mutations:

• F99S/M153T/V163A: GFPuv or cycle 3 GFP was optimised for excitation by UV light (360-400nm). [1]
• S208L: Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2]
• A206K: Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]

Notes

The start codon was replaced by a GSTGS linker to enable scarless C-terminal protein fusions.

Source

We started from pBAD-GFPuv (GenBank: U62637.1, Clontech), applied the described mutations and codon optimised the sequence for E. coli.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GSTGS linker	1,15	feature/protein, CDS
GFPuv2	16,729	CDS, feature/cds
stop	727,729	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1896007/1

Member of these Collections

• iGEM Parts Registry