BBa_K1897009

BBa_K1897009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1897009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ang Shi Hui

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 11:32:29

LuxI

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1897009_sequence (Version 1)

Description

This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, LuxI coding sequence BBa_C0061 and double terminator BBa_B0015.

Notes

There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag.

Source

LuxI was obtained from biobrick part BBa_C0061, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	13,67	engineered_region, feature/BioBrick
BBa_B0030	68,82	feature/BioBrick, engineered_region
BBa_C0061	95,706	feature/BioBrick, engineered_region
BBa_B0015	737,865	feature/BioBrick, engineered_region
HA tag	707,733	sequence_feature, feature/misc
Stop Codon	734,736	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1897009/1

Member of these Collections

• iGEM Parts Registry