Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fiona Tsai
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-19 09:49:33
Strong promoter + Strong RBS + CRYAB + 10x Histidine tag + Double terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1898450_sequence (Version 1) |
Description
BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The ORF includes cryab, a crystallin protein exists in the lens and other tissues, and 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.Notes
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. Primers were designed to remove the stop codon from cryab and were synthesized by Tri-I. Our assembly method BioBrick RFC[10] shifted the open reading frame and created a stop codon in between cryab and 10x histidine tag. The sequence was sent to Missiontech to add two base pairs in between the two genes.Source
the cDNA of cryab was ordered from OriGene. The promoter and rbs was from iGEM distribution kit (BBa_K880005) and His-Term was from an intermediate part we constructed (BBa_K1898500).| Access | Instance | Definition |
| public public public | BBa_K880005 BBa_K1898400 BBa_S05336 | BBa_K880005 BBa_K1898400 BBa_S05336 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K880005 BBa_K1898400 BBa_S05336 | 1,55 62,586 595,767 | BBa_K880005 BBa_K1898400 BBa_S05336 |