BBa_K1898550

BBa_K1898550 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1898550

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fiona Tsai

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-19 09:50:21

Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1898550_sequence (Version 1)

Description

BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.

Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. The double stop codon at the end of BBa_E0040 was also removed.

Source

This construct was designed and sent to IDT for oligo synthesis.

Components

Access	Instance	Definition
public	BBa_K880005	BBa_K880005
public	BBa_E0040	GFP
public	BBa_S05336	BBa_S05336

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K880005	1,55	BBa_K880005
BBa_E0040	62,781	GFP
BBa_S05336	790,962	BBa_S05336

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1898550/1

Member of these Collections

• iGEM Parts Registry