Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nienke Kuipers
Date created: 2009-09-07 11:00:00
Date modified: 2015-05-08 01:11:15
Arsenic Accumulation device (Low constitutive promoter + fMT + GlpF)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K190039_sequence (Version 1) |
Description
A device which can be used to accumulate As(III) without (under certain concentrations) toxifying E. coli. The expression of both genes is constitutively expressed (under low constitutive promoter).Notes
The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.Source
The device is composed from BBa_K190031, which contains the low constitutive promoter (BBa_J23109) together with fMT (BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.| Access | Instance | Definition |
| public public public | BBa_J23109 BBa_K190019 BBa_K190028 | BBa_J23109 BBa_K190019 BBa_K190028 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23109 BBa_K190019 BBa_K190028 | 1,35 44,265 272,1117 | BBa_J23109 BBa_K190019 BBa_K190028 |